Quantitative light microscopic autoradiography. Emulsion sensitivity and latent image fading.
نویسندگان
چکیده
منابع مشابه
Quantitative Electron Microscopic Autoradiography
Quantitative electron microscopic autoradiography was used for comparing the binding of labeled somatostatin-14 (S-14) and somatostatin-28 (S-28) to islet cells. Monolayer cultures of rat islet cells were incubated with ['251-Tyr"jS-14 (S-14) or [125I-Leu8, D-Trp22, TyreJS-28 (S-281) in the presence or absence of excess unlabeled peptides. Autoradiographic grains (ARG) associated with individua...
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In 18F micro-autoradiography using a frozen section method, the grain production was measured relative to the exposure time and radioactivity. The grain production increased until 6-hr exposure, but 7-hr or longer exposure induced a characteristic of latent image fading. However, the ratios of the efficiency of grain production between two different 18F-radioactivities (11.9 and 2.3 mBq/100 mic...
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An improved method has been developed for the preparation of autoradiographs for electron optical study. The refinement lies principally in the routine production of uniformly thin layers of photographic emulsion over the tracer-labeled cell sections. This is accomplished by means of a centrifugal spreading mechanism. Maize root tips grown in the presence of H(3)-thymidine were examined electro...
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Iodide is concentrated from the blood by the thyroid gland; after oxidation it is incorporated in tyrosyl residues of the thyroglobulin molecule. Thus the halogen is stored as a component of thyroglobulin in the colloid of the thyroid follicle. Secretion of thyroid hormone is considered to require proteolysis of the thyroglobulin and release of iodinated thyronines from the base of the acinar c...
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This collection of writings reflects the author's unique capacity to choose a topic, to mull over it gracefully, and to evoke the perplexing nature of a number of fundamental notions (and the sentiments which these themes engender) regarding the human experience, i.e., life and death. While the writing style and diction are often in and of themselves as complex and intricate as any textbook des...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1975
ISSN: 0022-1554,1551-5044
DOI: 10.1177/23.12.1194673